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. 2024 Feb 15;43(6):956–992. doi: 10.1038/s44318-024-00049-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV4 — (A) Fluorescence images showing expression of glb-26prom::gfp in wild type and unc-30(e191) mutants. (B) Fluorescence images showing expression of dmd-4prom::mCherry in wild type and unc-30(e191) mutants. Arrows point to ectopic expression in unc-30(e191) mutants. (C) Quantification of ectopic expression of the two HMC reporters shown in (A) and (B) in unc-30 mutant backgrounds. (D) Cells ectopically expressing (white arrow) the HMC reporter dmd-4prom::mCherry (magenta) always co-express let-381::gfp (green). (E) Expression of arg-1prom::gfp is lost in HMC in dmd-4(ot933) mutants. (F) Total number of GLR glia cells expressing either the GLR glia-specific nep-2prom7::rfp or the HMC-specific arg-1prom::gfp in unc-30(e191) mutants. Data information: unpaired t test used for statistical analysis in (C). Anterior is left, dorsal is up and scale bars are 10 μm for all animal images.