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. 2024 Feb 15;43(6):956–992. doi: 10.1038/s44318-024-00049-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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(A–D) Endogenous expression of (A) nep-2, (B) pll-1, (C) hlh-1, and (D) inx-18 in wild-type and let-381 motif-mutated animals (details on endogenous gfp reporters and molecular identity of let-381 motif mutations are shown in Fig. EV1F). Animal images are on the left. Dashed circles outline expression in GLR glia. Quantifications are shown in the dot plots on the right. Data information: Mean fluorescence expression intensity for each GLR glia cell for (A, B, D) and number of bright gap-junction puncta for (C) is compared between the wild-type and let-381-motif-mutated backgrounds. Black lines indicate averages. Unpaired t test used for statistical analysis. a.u. = arbitrary units. Anterior is left, dorsal is up, and scale bars are 10 μm for all animal images. Source data are available online for this figure.