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. 2024 Feb 15;43(6):956–992. doi: 10.1038/s44318-024-00049-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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(A) unc-30 genomic locus showing mutant alleles, reporters, fosmid genomic clones used in this study. (B) Expression of the endogenous unc-30::gfp reporter at different stages during development. Dashed red circles outline GLR glia. (C) nep-2prom7::gfp expression in a wild-type L4 (left) and a unc-30(e191) null mutant L4 animal (right). GFP expression is lost in the lateral and ventral GLR glia in unc-30(e191). The anterior process of the dorsal GLR glia still expressing GFP is shorter than that of a wild-type animal. (D) Quantification of percentage of each GLR glia cell with nep-2prom7::gfp expression (L4 stage) for different unc-30 mutant backgrounds. (E) Quantification of the length of GLR anterior processes (L4 stage) for different unc-30 mutant backgrounds. (F) Images of L4 animals showing differences in endogenous unc-30::gfp expression upon mutation of the let-381 motifs present in the fifth intron of unc-30. Dashed red circles outline GLR glia and black asterisks denote expression in the ASG and AVJ head neurons. (G) Quantification of unc-30::gfp fluorescence intensity for each GLR glia cell; black lines in bar graphs indicate averages. Data information: unpaired t test used for statistical analysis in (G). a.u. = arbitrary units. Anterior is left, dorsal is up, and scale bars are 10 μm for all animal images. Source data are available online for this figure.