FIG. 4.
Molecular characterization of epithelial raft cultures of FK16A cells at passages 20 and 81 and FK16B cells at passage 63. HPV16 E6/E7, in situ hybridization with 35S-labeled antisense riboprobes to reveal HPV16 E6-E7 transcripts. Hybridization of FK16 p20 was performed in a separate experiment. The signal intensities should not be compared between the two experiments, but relative signal strengths in cells at different strata within each culture represent valid comparisons. Immunohistochemical staining: MIB, Ki-67 expression; p21, p21cip1 protein expression.