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. 2024 Mar 15;15(3):218. doi: 10.1038/s41419-024-06594-w

Fig. 3. Influence of LIF on neutrophil recruitment and formation of NETs in the GC tumor microenvironment.

Fig. 3

A Levels of LIF in serum and ascites of both non-PM group (n = 12) and PM group (n = 18) GC patients; B Pearson analysis of the correlation between serum MPO-DNA and LIF levels in PM group (n = 18) GC patients, and the correlation between ascites MPO-DNA and LIF levels; C Representative H&E and immunofluorescence co-staining images of Cit-H3 and LIF in the greater omentum tissue of PM group GC patients, with Hochest staining for nuclei in blue, Cit-H3 staining in green, and LIF staining in red; D Schematic diagram of the recruitment of neutrophils by CM from AGC cells or MKN-45 cells overexpressing or interfering with LIF, or by CM from neutral neutrophils (Neu) pretreated with CM from these cancer cells for 12 h; E Neutrophil counts in each group of migration; F Immunofluorescence co-staining detection of Cit-H3 and MPO levels, with Hochest staining for nuclei in blue, Cit-H3 staining in green, and MPO staining in red. *P < 0.05, cell experiments were repeated at least three times.