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. 2024 Mar 15;15(3):218. doi: 10.1038/s41419-024-06594-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Levels of TGF-β in ascites of non-PM group (n = 12) and PM group (n = 18) GC patients; B Pearson analysis of the correlation between MPO-DNA in ascites of PM group (n = 18) GC patients and TGF-β levels; C Detection of LIF mRNA levels by RT-qPCR and protein expression of LIF, Smad2/3 by Western Blot; D ChIP-qPCR to detect potential binding sites of Smad2/3 in the LIF promoter region; E Dual-Luciferase reporter assay to investigate the transcriptional regulation of LIF by Smad2/3; F ELISA to measure the levels of LIF in GC cell culture medium. * Indicates that P < 0.05 and cell experiments are repeated at least three times.