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. 2024 Mar 15;15(3):218. doi: 10.1038/s41419-024-06594-w

Fig. 6. The in vivo induction of PM by NETs may be associated with the activation of LIF.

Fig. 6

A ELISA was used to measure the levels of LIF in serum and ascites of nude mice; B The neutrophil count in ascites of the PM nude mouse model was determined; C Flow cytometry was performed to analyze the proportion of neutrophils in ascites of nude mice; D ELISA was conducted to assess the levels of MPO-DNA complexes in the ascites of nude mice, indicating the levels of NETs; E Representative images of H&E and immunofluorescence staining of Cit-H3.MPO in the retinal tissue of nude mice, with cell nuclei labeled in blue, Cit-H3 in green, and MPO in red, scale bar = 50 μm; F Representative images and quantification of peritoneal metastatic lesions in PM nude mice. G Observation and statistical analysis of visible metastatic nodules on the mesentery of nude mice. N = 10.