FIGURE 2.

oCOm‐21 does not alter myofilament phosphorylation in a permeabilized cardiomyocyte. (a) Representative Pro‐Q™ Diamond protein phosphorylation‐stained gradient gel and, (b) subsequent SYPRO® Ruby total protein staining. (c) Ratio of Pro‐Q™ Diamond and SYPRO® Ruby signal for cTnI. (d) Ratio of Pro‐Q™ Diamond and SYPRO® Ruby signal for total protein phosphorylation. Individual data points in (c, d) are denoted using black solid circles. Data are expressed as a Box and Whiskers plot representing the median and interquartile range, n = 9 hearts/group. Significance was determined using a One‐way ANOVA with Dunnett post hoc test and p is represented on each experimental group.