FIG. 2.

RT-PCR detection of chemokine receptor expression in macrophages. RNA was extracted from 7-day-old macrophage cultures and subjected to reverse transcription and PCR amplification for CCR5, CXCR-4, CCR3, CCR8 (chemR1), and CCR2b, as well as CD4. Products were separated on agarose gels and stained with ethidium bromide. Macrophages from a CCR5 wild-type-homozygous donor are shown, and the same pattern was seen with cells from ccr5Δ32-homozygous donors. Amplification was done following reverse transcription with (+) or without (−) reverse transcription enzyme present. m, molecular size standards.