FIG. 3.

Macrophage surface expression of CXCR-4 by flow cytometry. Seven-day-old cultures of monocyte-derived macrophages (MDM) were detached and stained with MAbs for major histocompatibility complex class I as a positive control (w6/32), a T-cell marker (OKT3), a macrophage marker (OKM1), CD4 (#19), and CXCR-4 (12G5). Cells were then analyzed by flow cytometry with a minimum of 10⁴ cells. Specific MAb profiles are indicated by the black histogram, and the negative control antibody is shown as the shaded histogram in each graph. Macrophages from a CCR5 wild-type-homozygous donor are shown and are representative of cells from six different donors. The same patterns were seen with cells from ccr5Δ32-homozygous donors. The SUP-T1 cell line was stained in parallel.