REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
APC anti-human B2M antibody	Biolegend	Cat#316312; RRID: AB_10643412
APC anti-human CD4 antibody	Biolegend	Cat#357408; RRID: AB_2565659
FITC anti-human CD26 antibody	Biolegend	Cat#302704; RRID: AB_314288
FITC anti-human CD46 antibody	Biolegend	Cat#315304; RRID: AB_2291290
FITC anti-human CD47 antibody	Biolegend	Cat#323106; RRID: AB_756136
FITC anti-human CD55 antibody	Biolegend	Cat#311305; RRID: AB_314863
FITC anti-human CD59 antibody	Biolegend	Cat#304706; RRID: AB_2076126
PE anti-human CD63 antibody	Biolegend	Cat#353004; RRID: AB_10897809
APC anti-human CD81 antibody	Biolegend	Cat#349510; RRID: AB_2564021
FITC anti-human CD97 antibody	Biolegend	Cat#336306; RRID: AB_1227609
APC anti-human CD274 antibody	Biolegend	Cat#329708; RRID: AB_940360
PE anti-human LDLR antibody	BD Biosciences	Cat#565653; RRID: AB_2739325
Chemicals, peptides, and recombinant proteins
Benzonase (2000 U/mL)	Millipore Sigma	Cat#E1014
Effectene	QIAGEN	Cat#301427
vectashield	Vector Laboratories	Cat#H-1000
Deposited data
Pooled Golden Gate screen read counts	This manuscript	Data S3
Experimental models: Cell lines
Human A375 cells	Cancer Cell Line Encyclopedia	N/A
Human HT29 cells	Cancer Cell Line Encyclopedia	N/A
Human MelJuSo cells	Cancer Cell Line Encyclopedia	N/A
Human HEK293T cells	ATCC	ATCC CRL-3216
Drosophila melanogaster S2R+	Yanagawa et al., 199844	DGRC Stock 150; RRID:CVCL_Z831
Drosophila melanogaster S2R+-NPT005	Neumüller et al., 201246	DGRC Stock 229; RRID:CVCL_0Q79
Experimental models: Organisms/strains
Drosophila melanogaster nub-Gal4	Bloomington Drosophila Stock Center (BDSC)	RRID:BDSC_84288
Drosophila melanogaster yw, nos-phiC31;attP2	Bloomington Drosophila Stock Center (BDSC)	RRID:BDSC_25710
Drosophila melanogaster yw, nos-phiC31;attP40	Bloomington Drosophila Stock Center (BDSC)	RRID:BDSC_25709
Drosophila melanogaster yw; UAS-DjCas13-2A-GFP{attP2}	This paper	N/A
y w; Dr e/TM3, Sb	Perrimon Lab	N/A
y w; Gla/CyO	Perrimon Lab	N/A
Recombinant DNA
Fragmid Resource release 1	This manuscript	Addgene Kit 1000000237
Plasmid pBS130	Gohl et al., 201145	Addgene 26290
Plasmid pCFD3	Port et al., 201447	Addgene 49410
Plasmid pRDA_174	DeWeirdt et al., 202120	Addgene 136476
Plasmid pLX_311-Cas9	Doench et al., 201419	Addgene 96924
Plasmid pAdDeltaF6	unpublished	Addgene 112867
Plasmid pAAV2/6	unpublished	generously provided by Dr. Guangping Gao and Dr. Jun Xie of the UMass Chan Medical School Viral Vector Core
Plasmid pAAV-CAG-GFP	Mao et al., 201131	Addgene 28014
Software and algorithms
PoolQ	The Broad Institute	https://portals.broadinstitute.org/gpp/public/software/poolq
CRISPick	The Broad Institute	https://portals.broadinstitute.org/gppx/crispick/public
Fragmid	The Broad Institute	https://portals.broadinstitute.org/gppx/fragmid/public