TABLE 2.
Requirement of Rev/RRE for efficient vector productiona
| gag-pol plasmid | Rev expressionb | RT activity (mU/ml) | Mean titerc (LFU/ml of virus stock) ± SD |
|---|---|---|---|
| pGP-RRE1 | −d | 4 ± 3 | <1 |
| pGP-RRE1 | + | 3,400 ± 600 | (3.1 ± 0.0) × 105 |
| pGP-CTEr | + | 7 ± 1 | (2.0 ± 0.2) × 102 |
| pGP-CTE | + | 95 ± 4 | (3.0 ± 1.8) × 103 |
a
Viral stocks were generated by cotransfection of each gag-pol expression plasmid (7 μg), pH4Z (8 μg), and pRV67 (5 μg). Results are from a representative experiment of a total of three performed.
b
Rev is expressed from the vector genome, pH4Z.
c
Titer was measured on 293T cells by counting the number of blue colonies following X-Gal staining 48 h after transduction.
d
Instead of pHZ4, which is rev positive, the same amount of pCI-neo was transfected.