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“Apyrase chase.”A, scheme. B, time course of the ubiquitination of CK1α by E3 CRL4CRBN and UbcH5c. C, the “apyrase chase” reaction with CK1α, E3 CRL4CRBN, UbcH5c, and Cdc34b using the protocol described in the Experimental procedures section. Both high- and low-contrast fluorescent scans are shown. Presumed ubiquitin molecules linked to CK1α are as indicated by in-gel numbers. Percentage of CK1α consumed is indicated. CK1α, casein kinase 1α; CRL, Cullin–RING E3 Ub ligase.
