Elongation of ubiquitinated β-catenin with defined chain length.A, decay of β-catenin–Ub1. B, decay of β-catenin–Ub2. C, decay of β-catenin–Ub4. D, graph compares turnover rates of β-catenin–Ub1, β-catenin–Ub2, and β-catenin–Ub4 at time scale of 10–60 s. It integrates data in triplets with indicated error bars. The reaction was carried out using the protocol described in the Experimental procedures section. Ub, ubiquitin.