FIG. 1.
Permissiveness to HIV-1 infection increased as monocytes differentiated into MDM. Cells were infected with HIV-1 WM1101 primary isolate (or HIV-1BaL [data not shown]) either immediately after isolation (day 0 [D0]) or after culture on plastic for 1, 3, 7, and 10 days (D1, D3, D7, and D10, respectively). Replication kinetics of HIV-1 WM1101 by cultured monocytes was measured by determining amounts of the extracellular p24 antigen in culture supernatants (A) with an enzyme-linked immunosorbent assay commercial kit (Organon Teknika) and by quantitative hot PCR with 32P-labeled primer for the detection of full-length cDNA (320 bp) (panel B, first gel) or initiation products (140 bp) (panel B, third gel) of HIV reverse transcription. Culture supernatants were collected 0, 3, 7, and 14 days after infection for the detection of HIV p24 antigen. DNA was extracted from cells at 12 h and 1, 2, 3, 5, and 7 days after infection and used in hot PCR to detect HIV DNA. β-Globin DNA (110 bp) was amplified concurrently with HIV DNA, and results are shown underneath each DNA sample (panel B, second and fourth gels). p.a., postadherence; AZT, zidovudine.