Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 16;15:2384. doi: 10.1038/s41467-024-46602-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a qPCR of Malat1 in BMMs from Malat1^+/+, Malat1^−/−, and Malat1^−/−;Malat1^Tg/Tg mice. b TRAP staining (left) and quantification (right) of Malat1^+/+, Malat1^−/−, and Malat1^−/−;Malat1^Tg/Tg BMMs treated with M-CSF and RANKL. Scale bars, 125 μm (upper) and 50 μm (lower). n = 5 wells per group. c, d qPCR of Ctsk (c) and Trap5 (d) in the BMMs described in b. e qPCR of Malat1 in Malat1-knockdown RAW264.7 cells. f TRAP staining (left) and quantification (right) of RANKL-treated control and Malat1-knockdown RAW264.7 cells. Scale bars, 100 μm. n = 3 wells per group. g Left: RANKL-treated control and Malat1-knockdown RAW264.7 cells were stained with Phalloidin Green 488 and DAPI. Right: data quantification. Scale bars, 50 μm. n = 16, 14, and 7 cells per group. h, i qPCR of Ctsk (h) and Trap5 (i) in RANKL-treated control and Malat1-knockdown RAW264.7 cells. j, k Immunoblotting of Nfatc1, Ctsk, and β-actin in Malat1^+/+, Malat1^−/−, and Malat1^−/−;Malat1^Tg/Tg BMMs treated with M-CSF and RANKL (j), and in RANKL-treated control and Malat1-knockdown RAW264.7 cells (k). l, m qPCR of Nfatc1 in Malat1^+/+, Malat1^−/−, and Malat1^−/−;Malat1^Tg/Tg BMMs treated with M-CSF and RANKL (l), and in RANKL-treated control and Malat1-knockdown RAW264.7 cells (m). n The mouse Ctsk promoter. Primers previously reported for amplifying N1 and N3 regions were used for ChIP-qPCR. o, p ChIP-qPCR showing the occupancy of the N1 (o) and N3 (p) regions of the Ctsk promoter by Nfatc1 immunoprecipitated from RANKL-treated control or Malat1-knockdown RAW264.7 cells. q, r Control and Malat1-knockdown RAW264.7 cells were transfected with negative control (NC) or Nfatc1 siRNA. After 24 h, the cells were treated with RANKL for 5 days, followed by TRAP staining and quantification (q). Scale bars, 100 μm. Cell lysates were subjected to immunoblotting of Nfatc1, Ctsk, and β-actin (r). n = 3 wells per group. Statistical significance in a–i, l, m, and o–q was determined by a two-tailed unpaired t test. Error bars are s.e.m. n = 3 biological replicates in a, c–e, h, i, l, m, o, and p. The experiments in j, k, and r were repeated independently three times, yielding similar results. Source data are provided as a Source Data file.