TABLE 1.
HTLV-2 transformation efficiency and reproducibility
| Expt. | No. of antigen-positive culturesa/no. of wells (%) with LAMP/MO input cell number of:
|
||
|---|---|---|---|
| 10 | 100 | 1,000 | |
| A | 1/10 (10) | 2/10 (20) | 4/10 (40) |
| B | 2/8 (25) | 4/8 (50) | 6/8 (75) |
| C | 3/40 (8) | 22/30 (73) | 22/27 (81) |
| Total | 6/58 (10) | 28/48 (58) | 32/45 (71) |
a
Results are expressed as the number (%) of antigen-positive (p24 concentration >1,000 pg/ml) cultures over the total number of wells seeded that could be established as cell lines after cocultivation of 104 PBMCs with different cell concentrations (10, 100, and 1,000) of irradiated LAMP/MO.