. 1998 Jan;72(1):841–846. doi: 10.1128/jvi.72.1.841-846.1998

TABLE 2.

Cell surface phenotypes of HTLV-2 transformants

Phenotype^a	No. of cultures with indicated phenotype at LAMP/MO input cell no. of:			Total no. (%) of cultures with phenotype
Phenotype^a	10	100	1,000	Total no. (%) of cultures with phenotype
CD2⁺ or CD3⁺	2^b	19^c	20^d	41
CD4⁺	1	13	2	16 (39)
CD8⁺	0	0	2	2 (5)
CD4⁺ and CD8⁺	1	6	16	23 (56)

Cell lines were designated as CD4⁺ or CD8⁺ if >95% of the cells were positive for the subtype by flow cytometric analysis as described in the text. All HTLV-2-positive transformants in these experiments were B1 negative. The cell lines were phenotyped by one- or two-color fluorescence by using a FACScan (Becton-Dickinson and Co., Mountain View, Calif.) and were analyzed using the Lysys 2.0 software package. The following antibodies were used: CD2, CD3, CD4, CD8, B1, KC56 (T200 Ag), and MsIgG (mouse isotypic control containing immunoglobulin subtypes G1, G2a, G2b, G3) (Coulter Immunology).

From three HTLV-2-transformed cultures.

From 22 HTLV-2-transformed cultures.

From 24 HTLV-2-transformed cultures.