Fig. 1.

Screening of CPNE3 as a downstream target gene regulated by YAP1. A mRNA sequencing in BGC-823 cells with downregulated YAP1 expression. B Examination of mRNA expression by quantitative reverse transcription polymerase chain reaction (RT-qPCR) after downregulating YAP1 in BGC-823 cells. C, D mRNA expression levels of YAP1, CPNE3, and CYR61 in AGS and HGC-27 cells (transfected with NC, siYAP1-#1, and siYAP1-#2 siRNAs). E Two-dimensional visualization of CPNE3 and YAP1 in single-cell clusters in patients with gastric cancer (GC). F Protein expression levels of YAP1, CPNE3, and CYR61 following down-regulation of YAP1 expression in AGS and HGC-27 cells. G, H After gradient overexpression of Flag-YAP1, the YAP1, CPNE3, and CYR61 proteins and mRNA levels were detected by western blotting (WB) and RT-qPCR, respectively. Three independent biological experiments were performed, and statistical significance is denoted by * p < 0.05 and ** p < 0.01