Fig. 2.

Direct regulation of CPNE3 expression by YAP1/TEAD4. A CPNE3 mRNA levels were analyzed with TEAD1 and TEAD4 expression using the GEPIA database. B Two-dimensional visualization of CPNE3 and TEAD1 or TEAD4 in single-cell clusters in patients with GC. C, D CPNE3 mRNA was detected by RT-qPCR, and expression of CPNE3 and YAP1 by WB with appropriate antibodies as indicated in AGS cells stably expressing YAP1-WT, YAP-5SA, or YAP-S94A. E, F YAP1-WT plasmid was introduced into TEADs-deficient BGC-823 cells, and CPNE3 mRNA expression and protein expression levels were detected by RT-qPCR and WB. G The CPNE3 promoter was cloned into a luciferase reporter to verify the effect of YAP1 and TEAD1/2/3/4 on CPNE3 transcriptional activity. H The JASPAR database yielded several possible TEAD4 binding sites on the CPNE3 promoter. I Three primers created for the aforementioned TEAD4-binding sites were used for chromatin immunoprecipitation