Fig. 3.

Mechanism of CPNE3 in promoting proliferation and drug resistance in vitro in GC cells. A The proliferation of BGC-823 and MKN-28 cells was significantly inhibited when CPNE3 was downgraded using siCPNE3-#1 or siCPNE3-#2, as shown by the CCK-8 assay. B–D Transwell assay and clone formation assay revealed suppressed cell migration, invasion, and colony formation ability of GC cells. E, F In vitro chemosensitivity tests using gradient concentrations of 5-fluorouracil or docetaxel showed that downregulation of CPNE3 enhanced the sensitivity of GC cells to chemoresistant drugs. G–J HA-CPNE3 plasmids were stably transfected into AGS and HGC-27 cells, and cell function tests demonstrated that HA-CPNE3 significantly increased GC cell proliferation, migration, invasion, and colony formation ability. Three independent biological experiments were conducted, which consistently yielded similar results. Statistical significance is indicated by *p < 0.05 and **p < 0.01. Scale bar: 200 μm