Fig. 4.

CPNE3 is associated with the Hippo-YAP1 pathway in GC. A Enrichment analysis of CPNE3 with Hippo-YAP1. B Pearson correlation analyses of CPNE3 with Hippo-YAP1 C–E Using siRNA, the expression of CPNE3 at the mRNA and protein levels were downregulated in BGC-823 and MKN-28 cells. F, G Up-regulation of CPNE3 expression by transfection of HA-CPNE3 plasmid in AGS and HGC-27 cells. H WB revealed that the expression of YAP1 and its target gene CYR61 were both considerably upregulated in AGS and HGC-27 cells using the same technique used to boost CPNE3 expression as above. I, J Confocal images of HA-CPNE3 plasmid transfection in HGC-27 cells and siCPNE3-#1 or siCPNE3-#2 transfection in BGC-823 cells with YAP1 labeling