Fig. 6.

CPNE3 promotes GC progression in a partial YAP1-dependent manner. BGC-823 and MKN-28 cells were treated with the ShCPNE3-#2 plasmid to downregulate the expression of CPNE3 and the Flag-YAP1 plasmid to concurrently increase the expression of YAP1. A WB was used to measure the expression of CPNE3, YAP1, and CYR61. B–G Phenotyping assays were used to determine the degree to which the CPNE3 depletion-induced suppression of GC cell proliferation, migration, invasion, colony formation, and drug resistance could be reversed by the overexpression of exogenous YAP1. H–K The capacity of MKN-45 cells to proliferate, invade, migrate, and form colonies was weakly inhibited by downregulation of CPNE3 expression in MKN-45 cells lacking YAP1 expression. ShYAP1-#1 plasmid-mediated stable YAP1 knockdown or HA-CPNE3 plasmid-mediated simultaneous overexpression of CPNE3 in AGS and HGC-27 cells. L WB was used to investigate the relevant protein levels. M–P The overexpression of CPNE3 did not alleviate the suppression of GC cell proliferation, migration, invasion, and colony formation brought on by the downregulation of YAP1. Three independent biological experiments were conducted, and statistical significance is shown by the notations, *p < 0.05 and **p < 0.01