Fig. 7.

CPNE3 promotes GC growth in vivo. A, B Cell line with stable silencing of CPNE3 expression was established in BGC-823 cells by infection with lentivirus encoding sgRNA targeting CPNE3 or negative control. C–E Silencing of CPNE3 significantly reduced tumor growth in vivo, and the weight and volume of the tumor tissues were significantly lower than those in controls (mean ± standard error of the mean [SEM] n = 10/group). F, G Immunohistochemistry (IHC) and WB tests were used to assess the protein levels of CPNE3, YAP1, and CYR61 in tumor tissues from subcutaneous cell line-derived xenograft models constructed from BGC-823 cells with CPNE3 expression downregulated by lentivirus. H–J Using the same method described above to validate the function of CPNE3 in a patient-derived xenograft (PDX) model of GC, silencing CPNE3 significantly reduced tumor growth, weight, and volume in vivo (mean ± SEM n = 6/group). K, L Protein expression of CPNE3, YAP1, and CYR61 were detected using WB and IHC, assays in BGC-823 cells after stably downregulating CPNE3 expression in the PDX model