Fig. 8.

CPNE3 is an independent prognostic factor that causes poor prognosis in patients with GC. A Using Western blotting, the protein levels of CPNE3 in eight pairs of GC patient tissue samples were measured. B CPNE3 protein expression was examined the IHC of tumor tissues (n = 20) and matched normal tissues (n = 20) from patients with GC. C–E Overall survival and CPNE3 expression were used to stratify the patients in the training, validation, and training + validation groups, followed by Kaplan–Meier analysis. F The chi-square test was used to determine the relationship between the expression of CPNE3 and that of YAP1, CYR61, and RAD51. G Kaplan–Meier survival analysis was performed in 100 patients who were categorized based on their CPNE3 and YAP1 protein levels. H Diagram by Figdraw showing the process through which the CPNE3-YAP1 positive feedback loop promotes GC