Figure 2.

Stoichiometry of complex of HopQ1 with 14-3-3a. Recombinant 14-3-3a protein with a C-terminal Strep II epitope (A) and in vitro reconstituted HopQ1:14-3-3a complex (B) were subjected to gel filtration coupled with MALS analyses. Blue and red traces correspond to absorption at 280 nm and 254 nm, respectively; green traces indicate light scattering (LS) at 90° angle and black indicates molecular weights. Comparison of the theoretical molar masses for HopQ1, 14-3-3a and HopQ1:14-3-3a complex with the respective MALS derived molar masses is shown in the table. (C) Mid-peak fraction corresponding to HopQ1:14-3-3a complex was fractionated on 12.5% SDS-Page and stained with Coomassie Brilliant Blue.