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. 2024 Mar 4;15:1335830. doi: 10.3389/fpls.2024.1335830

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Copyright © 2024 Rymaszewski, Giska, Piechocki, Zembek and Krzymowska

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dynamics of HopQ1 flow between the cytoplasm and nucleus. HopQ1 variants fused to photoconvertible mDendra2 protein were transiently expressed in N. benthamiana cells. Green-to-red photoconversion was achieved by laser stimulation of a defined spot in the cytoplasm from 20 sec of imaging onward. Red fluorescence intensity was recorded in the cytoplasm and nuclei of 50-70 cells per variant during 6 consecutive experiments. Curves for both measured cellular compartments show changes in red fluorescence intensities normalized by the final intensity in the cytoplasm. Areas under curves (AUCs) were analyzed statistically with an ANOVA and Tukey’s HSD test and the slope (starting with 50 s) with Mann-Whitney test (***p < 0.001).