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Buffer composition.
| Name of buffer | Ingredients | Final concentration |
|---|---|---|
| Digestion mix | RPMI (with glutamine) | |
| Collagenase IV | 200U/mL | |
| 0.5 U/mL DNase I | 0.5U/mL | |
| PBS/EDTA solution | PBS | |
| EDTA | 2mM | |
| FACS buffer | PBS | |
| FCS | 3% | |
| EDTA | 2mM | |
| DSS solution | Autoclaved water | |
| DSS | 2.5% |
