Table 2.
Reproducibility, background level and distribution of virus-specific IgG levels .
| Reproducibility | Background level |
Distribution in all participants included, median [IQR] | Distribution in participants with longitudinal data (measured in EGEA1 and EGEA2), median [IQR] | ||||
|---|---|---|---|---|---|---|---|
| mean CV | ICC | EGEA1 - children | EGEA2 – adults | EGEA1- children | EGEA2 – adults | ||
| (n=530) | (n=1241) | (n=270) | (n=270) | ||||
| RSV | 0.21 | 0.93 | 75.2 | 22473 [12110-35537] |
21914 [11317-37496] |
23065 [11831-36510] |
18132 [8041-33241] |
| RV-A | 0.12 | 0.97 | 67.1 | 885068 [604924-1060300] |
346732 [11317-37496] |
896054 [594349-1054170] |
581326 [271219-895467] |
| RV-B | 0.12 | 0.99 | 138.6 | 322210 [204258-437388] |
179646 [71527-328715] |
318204 [205738-444790] |
217956 [114922-383097] |
| RV-C | 0.12 | 0.97 | 74.2 | 293043 [164379-440619] |
86343 [31524-195329] |
281459 [158782-449288] |
135255 [53365-305127] |
Reproducibility was assessed from samples of 4 individuals of EGEA2 survey which were measured on each of the 6 arrays. For each virus-specific IgG level, the mean of the CV calculated for each of the four individuals is reported. Distributions of virus-specific IgG data are presented as median [interquartile range] of the calibrated and corrected for the background level values (before normalization) and expressed in FI.
IgG, Immunoglobulin G; CV, coefficient of variation; ICC, intra-class correlation coefficient; EGEA, Epidemiological study on the Genetics and Environment of Asthma; RSV, respiratory syncytial virus; RV, rhinovirus; FI, fluorescence intensity.