Fig. 3.

GADD45B up-regulation inhibits bladder cancer cell invasion and migration. (A) The protein expression level of GADD45B in various bladder cancer cell lines (T24, UMUC3, 5637, TCCSUP and J82) was assessed using western blot analysis. (B) Introduction of a lentiviral vector containing GADD45B significantly augmented the expression level of GADD45B in J82 cells. (C) Transfection of T24 cells with a shGADD45B lentiviral vector resulted in down-regulation of GADD45B protein expression. (D.E) Following modulation of GADD45B expression in J82 and T24 cells, tumorigenesis was evaluated through colony formation assays, involving a 10-day incubation period followed by crystal violet staining. (F.G) The cell invasion ability was assessed using the matrigel transwell assay. (H.I) The cell migration ability was measured by wound-healing assays. The GADD45B over-expressed group exhibited a substantial reduction in tumorigenesis, invasion, and migration abilities, whereas the GADD45B depleted group displayed a significant increase in these abilities. All data presented represent the mean of three independent experiments, with the standard error of the mean (SEM) indicated.