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. 2024 Mar 7;10(6):e27427. doi: 10.1016/j.heliyon.2024.e27427

Fig. 5.

Fig. 5

Down-regulation of GADD45B shifts bladder cancer cells from mitochondrial oxidative phosphorylation to glycolysis. (A) (B) The Seahorse XF mitochondrial stress test was used to measure basal and ATP-linked oxygen consumption following modulation of GADD45B expression. Up-regulation of GADD45B in J82 cells resulted in increased basal and ATP-linked oxygen consumption, whereas down-regulation of GADD45Bin T24 cells had the opposite effects. (C) (D)Up-regulation of GADD45B in J82 cells led to a significant decrease in glucose-6-phosphate and pyruvate level, while down-regulation of GADD45B in T24 cells also had the opposite effect. (E)(F) Western blot analysis revealed the presence of HK2 and PKM2 proteins. Notably, a negative correlation was observed between the expression levels of these proteins and GADD45B. All of the data show an average of three independent experiments ± SEM.