Ability of gH mutants to mediate fusion. (Top panel) Diagram of mutant gH proteins containing insertions at amino acid residues 691, 791, and 799. (Bottom panel) The total number of nuclei in polykaryocytes with more than 10 nuclei was scored on 4-cm2 areas transfected with plasmids expressing gB, gD, and gL, together with either wild-type or mutant gH proteins. The mean scores of two independent observers are plotted.