Fig. 5.

Effect of DIO on colonic TNF-α immunostaining changes (DAB, IHC, TNF-α, x400) in normal control (A), 4% (w/v) DSS-induced colitis (B), DSS-induced colitis treated with SSZ (200 mg/kg) (C), DSS-induced colitis treated with DIO at doses of 100 mg/kg (D), and 200 mg/kg (E), and (F) % of TNF-α immunostaining. DSS: dextran sulfate sodium; SSZ: sulfasalazine; DIO: diosmin; TNF-α: Tumor necrosis factor-alpha. Black arrows indicated mucosal epithelial lining cells and red arrows indicated number of lamina propria mononuclear cell. The values are presented as means of 6 mice ± SEM. One-way ANOVA was used for statistical analysis, followed by Tukey's post hoc test. *^{, #, †, ‡} Significantly different from normal, DSS-induced colitis, DSS + SSZ or DSS + DIO 100 mg/kg groups at p < 0.05, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)