REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
TSG101	Proteintech	Dilution (1:1,000); Clone: N/A; Cat# 14497-I-AP; RRID:AB_2208090
CD63	Proteintech	Dilution (1:1,000); Clone: N/A; Cat# 25682-I-AP; RRID:AB_2208090
Alix	Santa Cruz Biotechnology	Dilution (1:1000); Clone: 1A12; Cat# sc-53540; RRID:AB_673819
TSG101	Thermo Fisher Scientific	Dilution (1:1,000); Clone: 4A10; Cat# MA1-23296; RRID:AB_2208088
Anti-rabbit HRP	Cell Signaling Technology	Dilution (1:1,000);; Cat#: 7074S: RRID:AB_2099233
Anti-mouse HRP	Cell Signaling Technology	Dilution (1:1,000); Cat#: 7076P2: RRID:AB_330924
GFP	Santa Cruz Biotechnology	Dilution (1:1,000); Clone: NA; Cat# Sc-9996; RRID:AB_627695
SPHK1	Proteintech	Dilution (1:1,000); Clone: N/A; Cat# 10670-I-AP; RRID:AB_2195809
Zombie UV fixable viability kit	BioLegend	Dilution (1:1,000); Cat#: 423107
Anti-mouse CD4-BV711	BioLegend	Dilution (1:100); Clone: GK1.5; Cat# 100447; RRID: AB_2564586
Anti-mouse CD8-BV605	BioLegend	Dilution (1:100); Clone: 53-6.7; Cat# 100744; RRID:AB_2562609
Anti-mouse CD45-BV510	BioLegend	Dilution (1:100); Clone: 30-F11; Cat# 103138; RRID:AB_2563061
Anti-mouse CD3-BUV395	BD Horizon	Dilution (1:100); Clone; 145-2C11 (RUO) Cat# 563565; RRID:AB_2738278
Anti-mouse PD1-PE	BD Pharmingen	Dilution (1:100); Clone: N/A; Cat# 561788; RRID:AB_10895570
Anti-mouse TIM3-BV421	BD Biosciences	Dilution (1:100); Clone: 5D12; Cat# 747626; RRID:AB_2744192
Chemicals, peptides, and recombinant proteins
RIPA buffer	Thermo Fisher Scientific	Cat# 89901
Halt protease and phosphatase inhibitor cocktail	Thermo Fisher Scientific	Cat# 78444
Pierce BCA assay protein kit	Thermo Fisher Scientific	Cat# 23225
ECL Prime western blotting detection reagent	Thermo Fisher Scientific	Cat# 34580
G418	Thermo Fisher Scientific	Cat# J63871-AB
ACK lysis buffer (RBC lysis buffer)	Quality Biological	Cat# 118-156-101
FACS buffer	Tonbo Biosciences	Cat# 4222-L500
Cyto-Fast Fix/Perm buffer	BioLegend	Cat# 426803
TF Perm/Wash buffer (5X)	BD	Cat# 562725
Polybrene	Sigma	Cat# 107689
Bovine serum albumin (BSA)	Sigma	Cat# A3059
Opti-MEM (1X)	Gibco	Cat#31985-070
Dulbecco’s modified Eagle’s medium (DMEM)	Thermo Fisher Scientific	Cat# 10569010
ITS	Thermo Fisher Scientific	Cat# 41400045
Fetal bovine serum (FBS)	Atlanta Biologicals	Cat# H17112
Hanks' balanced salt solution (HBSS)	Atlanta Biologicals	Cat# 14170120
Antibiotic (penicillin/streptomycin)	Thermo Fisher Scientific	Cat# 15140122
Lipofectamine 2000	Thermo Fisher Scientific	Cat# 11668027
Vybrant DiI cell-labeling solution	Thermo Fisher Scientific	Cat# V22885
DPBS	Sigma	Cat#D8662
psPAX2	Addgene	Cat#12260
pMD2.G	Addgene	Cat#12259
pLCP-GFP	Addgene	Cat#17448
pLCP-GFP-SPHK1	Genecopoeia	Cat#EX-Mm05392-M68
Experimental models: Cell lines
293T	ATCC	Cat# CRL-3216
ID8	Sigma	Cat# SCC145
Experimental models: Organisms/strains
4–6-weeks-old female C57BL/6 mice	Envigo	-
Software and algorithms
GraphPad Prism 9.5	GraphPad	https://www.graphpad.com/
FlowJo v10.10	FlowJo LLC	https://www.flowjo.com/
NTA software 3.1	Malvern, Worcestershire, United Kingdom	https://www.malvernpanalytical.com
Others
Insulin syringe (31G)	BD	Cat# 328820
Centrifuge tube polypropylene (38.5 mL)	Beckman Coulter	Cat# 355642
PVDF	Bio-Rad	Cat #1620177
40 μM strainer	Greiner	Cat#542040
27G x 1/2 needle	BD	Cat# 305109
18G needle	BD	Cat#305136
NanoSight LM10	Malvern, Worcestershire, United Kingdom	https://www.malvernpanalytical.com