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. 2024 Feb 15;300(3):105759. doi: 10.1016/j.jbc.2024.105759

Figure 2.

Figure 2

RFFL downregulates Ito,fin HEK cells stably expressing Kv4.3 in a RING domain–dependent manner independently of KChIP2.A, representative traces of Ito,f in Kv4.3-expressing HEK cells transiently expressing GFP (control), RFFL and GFP, or RFFL-ΔRING and GFP. Top inset shows voltage protocol. Bottom inset shows cartoon of the constructs. B, cumulative I-V curves of Ito,f in Kv4.3-expressing HEK cells transiently expressing GFP (control, green lines and symbols), RFFL and GFP (red), RFFL-ΔRING and GFP (blue), in the presence of KChIP2 and DsRed (solid lines and symbols) and the absence of KChIP2 but with DsRed (dashed lines and open symbols). The first number in parentheses in the legend corresponds to the number of experiments (N) and the second number to the total number of the cells for each condition (n). A mixed effects model for repeated measures data test indicates statistically significant differences between the I-V curves, where ∗ corresponds to p < 0.05, ∗∗ −p < 0.01, ∗∗∗ −p < 0.001. ARbCM, adult rabbit ventricular cardiomyocyte; HEK, human embryonic kidney; KChIP2, Kv channel-interacting protein 2; RFFL, RING finger and FYVE-like domain–containing E3 ubiquitin protein ligase.