Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jan 23;4(1):ycae016. doi: 10.1093/ismeco/ycae016

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Representative FISH micrographs showing the presence of intracellular bacteria in Acanthamoeba trophozoites investigated in this study. Probes EUK516 conjugated with Cy5, targeting Acanthamoeba, and EUB conjugated with Cy3, targeting most of bacterial strains were used for all Acanthamoeba strains positive for bacterial 16S rRNA. DAPI was used in mounting medium when visualized by a fluorescence microscope. Probe pB-914 labelled with 6-FAM was used for isolates containing high abundance of bacteria belong to Enterobacteriaceae family. (A) Rod shaped bacteria were observed throughout the cytoplasm of Acanthamoeba trophozoites (Indian corneal isolate) and a few cocci bacteria were also observed (yellow arrows). The white arrow represents bacterium cell undergoing binary fission. (B) Bacteria showing binary fission (white arrows) in vacuole like structure of Acanthamoeba recovered from water sample (R3). (C and D) Corneal isolates of Acanthamoeba spp. (Ac-112 and L-579/20, respectively) with intracellular bacteria. (E) Intracellular bacteria labelled with probes EUB and pB-914 simultaneously in Acanthamoeba sp. isolated from an AK patient. (F) Clinical (Ac-102) isolate of Acanthamoeba trophozoite depicting rod shaped intracellular bacteria. Indicators: white arrow, bacterial cell undergoing binary fission; yellow arrow: cocci shaped bacteria. Scale bar in each panel represents 10 μm.