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. 2024 Jan 8;12:RP89489. doi: 10.7554/eLife.89489

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Recombinant DNA reagent FL BTK-C6H pET20 (plasmid) DOI: 10.1016/j.str.2017.07.014 Residues 1–659 wIth Y617P mutation, UniProt: P35991
Recombinant DNA reagent FL BTK-C6H Q91A, I92A, I94A, and I95A pET20 (plasmid) This paper Residues 1–659 wIth Y617P mutation
Recombinant DNA reagent FL BTK-C6H A384P, S386P, T387P, and A388P pET20 (plasmid) This paper Residues 1–659 wIth Y617P mutation
Recombinant DNA reagent FL BTK-C6H A384P, S386P, T387P, A388P, and L390F pET20 (plasmid) This paper Residues 1–659 wIth Y617P mutation
Recombinant DNA reagent FL BTK-C6H L542M, S543T, V555T, R562K, S564A, and P565S pET20 (plasmid) This paper Residues 1–659 wIth Y617P mutation
Recombinant DNA reagent SH3-SH2-kinase BTK-C6H pET20 (plasmid) This paper Residues 214–659 wIth Y617P mutation
Recombinant DNA reagent SH3-SH2-kinase BTK-C6H A384P, S386P, T387P, A388P, and L390F pET20 (plasmid) This paper Residues 214–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-FL BTK pET20 (plasmid) This paper Residues 1–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-FL BTK W251K pET20 (plasmid) This paper Residues 1–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-FL BTK R133E/Y134E/R171E pET20 (plasmid) This paper Residues 1–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-FL BTK R133E/Y134E/R171E/W251K pET20 (plasmid) This paper Residues 1–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-FL BTKsc pET20 (plasmid) This paper Residues 1–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-FL BTKsc Δ185–194 pET20 (plasmid) This paper Residues 1–184, 195–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-FL BTKsc Δ185–206 pET20 (plasmid) This paper Residues 1–184, 207–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-FL BTKsc Δ181–206 pET20 (plasmid) This paper Residues 1–180, 207–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-FL BTKsc Δ175–210 pET20 (plasmid) This paper Residues 1–174, 211–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-FL BTKsc Δ173–215 pET20 (plasmid) This paper Residues 1–172, 216–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-1PHTH171-G(GGSGG)4G-396kinase659 Q91A, I92A, I94A, I95A, K430R, L542M, S543T, V555T, R562K, S564A, and P565S pET28 (plasmid) This paper Residues 1–171, 396–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-1PHTH171-G(GGSGG)6G-396kinase659 Q91A, I92A, I94A, I95A, K430R, L542M, S543T, V555T, R562K, S564A, and P565S pET28 (plasmid) This paper Residues 1–171, 396–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-1PHTH176-G(GGSGG)4G-384kinase659 pET28 (plasmid) This paper Residues 1–176, 384–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-1PHTH176-G(GGSGG)4G-384kinase659 R133E pET28 (plasmid) This paper Residues 1–176, 384–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-1PHTH176-G(GGSGG)4G-384kinase659 Y134E pET28 (plasmid) This paper Residues 1–176, 384–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-1PHTH176-G(GGSGG)4G-384kinase659 R133E/Y134E pET28 (plasmid) This paper Residues 1–176, 384–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO-1PHTH176-G(GGSGG)4G-384kinase659 R133E/Y134E/171E pET28 (plasmid) This paper Residues 1–176, 384–659 wIth Y617P mutation
Recombinant DNA reagent N6H-SUMO KD Y551E/L390G pET20 (plasmid) This paper Residues 382–659 wIth Y617P mutation
Recombinant DNA reagent pCDFDuet YopH Thomas E. Smithgall
Strain, strain background (Escherichia coli) BL21 (DE3) Thermo Fisher Scientific  Cat#C600003 https://www.thermofisher.com/order/catalog/product/C600003
Other 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) Avanti Polar Lipids  Cat#850375P https://avantilipids.com/product/850375
Other 1,2-Dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) Avanti Polar Lipids  Cat#840035P https://avantilipids.com/product/840035
Other 1,2-Dioleoyl-sn-glycero-3-phosphoinositol-3,4,5-trisphosphate (tetra-ammonium salt) (PIP3) Avanti Polar Lipids  Cat#850156P https://avantilipids.com/product/850156
Other Pyruvate Kinase/Lactic Dehydrogenase MilliporeSigma  Cat#P0294 https://www.sigmaaldrich.com/US/en/product/sigma/p0294?gclid=EAIaIQobChMIobDx8MqZgwMVyVdyCh1-egAIEAAYASAAEgLOzPD_BwE
Other SYPRO Protein Gel Stains Thermo Fisher Scientific  Cat#S6650 https://www.thermofisher.com/order/catalog/product/S6650
Other Phusion Hot Start II DNA Polymerase Thermo Fisher Scientific  Cat#F549L https://www.thermofisher.com/order/catalog/product/F549L?SID=srch-srp-F549L
Antibody BD Pharmingen Purified Mouse anti-Btk (pY551)/Itk (pY511) (mouse monoclonal) BD Biosciences  Cat#558034 WB (1:1000)
Software, algorithm Coot DOI: 10.1107/S0907444910007493  v0.9.8.8 https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Software, algorithm autoPROC DOI: 10.1107/S0907444911007773  v20211020 https://www.globalphasing.com/autoproc/
Software, algorithm XDS DOI: 10.1107/S0907444909047337  v20220110 https://xds.mr.mpg.de/
Software, algorithm STARANISO DOI: 10.1107/S010876731809640X  v3.350 https://staraniso.globalphasing.org/staraniso_about.html
Software, algorithm CCP4i suite DOI:10.1107/S2059798323003595  v0.8 https://www.ccp4.ac.uk/
Software, algorithm Phaser DOI: 10.1107/S0021889807021206  v2.8 https://www.phaser.cimr.cam.ac.uk/index.php/Phaser_Crystallographic_Software
Software, algorithm Phenix DOI: 10.1107/S2059798319011471  v1.20-4459 https://phenix-online.org/
Software, algorithm CryoSparc Structura Biotechnology Inc  v4.1.2 https://cryosparc.com/
Software, algorithm PyMOL Shrodinger LLC  v2.5.5 https://www.pymol.org/
Software, algorithm DynamX WATERS  v3.0 https://www.waters.com/nextgen/us/en.html
Software, algorithm PLGS WATERS  v3.0 https://www.waters.com/nextgen/us/en.html
Software, algorithm FrameSlice The SIBYLS Beamline  N/A https://sibyls.als.lbl.gov/ran
Software, algorithm BioXTAS RAW DOI: 10.1107/S1600576717011438  v2.1.4 https://bioxtas-raw.readthedocs.io/en/latest/
Software, algorithm ATSAS: ALMERGE EMBL Hamburg  v3.2.1 https://www.embl-hamburg.de/biosaxs/manuals/almerge.html
Software, algorithm Chimera UCSF  v1.15 https://www.cgl.ucsf.edu/chimera/