Table 2. Calculated on-rates and off-rates for simulations presented in 1—5Figures 1—5.

On-rate is calculated using the biochemical k_on and the concentration of tubulin [µM]. Off-rate is calculated using the biochemical k_on and the dissociation constant K_D.

	Simulated end	On-rate (s–1)	kofflong (s–1)	koffcorner(s–1)	kofflong GDP (s–1)	koffcorner GDP (s–1)	Tubulin(µM)
Figure 1	plus	1	100	0.1	3x105	300	1
	minus	1	100	0.1	3x105	300	1
Figure 4A	plus	0.9	64	0.019	2.2x105	65	1.25
	minus	0.4	27	0.0078	9.3x104	27.3	1.25
Figure 4B	plus	0.9	64	0.019	2.2x105	65	1.25
Figure 4C	minus	0.4	27	0.0078	9.3x104	27.3	1.25
Figure 5B	plus	0.9	64	0.019	2.2x105	65	1.25