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. 2024 Jan 9;12:RP90136. doi: 10.7554/eLife.90136

Figure 1. Notch signaling is activated in newborn L4 neurons but not L5.

(A) Schematic of Notch signaling pathway. (B-B”) Hey as a reporter of active Notch signaling is only expressed in newborn L4 neurons but no other lamina neurons at 15 hr APF. Here and below, scale bar: 10 µm, n≥5 brains. Dashed line delineates the boundary between Bsh+ L4 and Bsh+ L5 neurons. (C, D) Hey is expressed prior to the activation of the secondary HDTFs Ap and Pdm3 at 15 hr APF. The dashed line delineates the boundary between Bsh+ L4 and Bsh+ L5 neurons. (E–H) Using twin-spot MARCM, two sibling neurons generated by one progenitor are traced. RFP and GFP cells are either both L4 neurons (Bsh+Pdm3-) or both L5 neurons (Bsh+Pdm3+). N=4. The dashed line outlines RFP+ and GFP+ cell bodies.

Figure 1.

Figure 1—figure supplement 1. E(spl)-mγ is not expressed in lamina neurons.

Figure 1—figure supplement 1.

(A-A”) E(spl)-mγ-GFP is expressed in the LPC region but not in lamina neurons. Erm labels L3; Svp labels L1; Bsh labels Bsh+ LPCs, L4 and L5. A solid white line outlines the LPC region.