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. 2024 Jan 9;12:RP90136. doi: 10.7554/eLife.90136

Figure 3. Bsh without Notch signaling activates Pdm3 and specifies L5 neuronal fate.

(A–D) N-KD in lamina (27G05-Gal4, tubP-GAL80[ts], UAS-Notch-RNAi) shows Hey expression is absent, and Bsh only activates Pdm3 and specifies L5 neuronal fate during lamina neurogenesis (19 hr APF). Here and below, scale bar, 10 µm, n≥5 brains. (E–J) N-KD in lamina shows Bsh+ lamina neurons are mainly Pdm3+ L5 neurons, though the number of Bsh+ lamina neurons remains unaffected at 3-4d APF. (I–J) Quantification (single optical section). (K) Summary Data are presented as mean ± SEM. Each dot represents each brain. n=6 brains in (I), (J). ***p<0.001, ns = not significant, unpaired t-test.

Figure 3.

Figure 3—figure supplement 1. 27G05-Gal4 is inserted in between two Notch target genes; Bsh without Notch signaling activates Pdm3 and specifies L5 morphology.

Figure 3—figure supplement 1.

(A) Schematic of 27G05-Gal4 insertion site in between two Notch target genes (E(spl)m4-BFM and E(spl)m5-HLH). (B-C”) Bsh without Notch signaling activates Pdm3 and specifies L5 morphology. In N-KD (27G05-Gal4, tubP-GAL80[ts], UAS-Notch-RNAi), Bsh activates Pdm3 but not Ap and specifies L5 neuronal fate during lamina neurogenesis (19 hr APF). The white line outlines Bsh+ lamina cells. Scale bar, 10 µm, n≥5 brains.
Figure 3—figure supplement 2. Bsh specifies L5 neuronal fate over L4 following Delta-KD.

Figure 3—figure supplement 2.

(A–C) Delta-KD (27G05-Gal4, tubP-GAL80[ts], UAS-Delta-RNAi) in lamina shows the increase of Pdm3+ L5 neurons at the expense of L4 at 3-4d APF. (C) Quantification (single optical section). Scale bar, 10 µm. Data are presented as mean ± SEM. Each dot represents each brain. n=5 brains. **p<0.01, ns = not significant, unpaired t-test.