Table 1. Cryogenic electron microscopy (cryo-EM) data collection and model refinement statistics.
| Data collection | |
|---|---|
| Microscope | FEI Titan Krios |
| Magnification | 165,000 |
| Voltage (kV) | 300 |
| Electron exposure (e-/Å2) | ~40 |
| Defocus range (µm) | –2.6 to –3.8 |
| Pixel size (Å) | 0.82 |
| Symmetry imposed | C5 |
| Number of images | ~18,982 |
| Particles picked | ~2.7 million |
| Particles refined | 16,586 |
| Refinement | |
| Resolution (Å) | 2.9 |
| FSC threshold | 0.143 |
| Map sharpening B-factor | –80 |
| Model composition | |
| Non-hydrogen protein atoms | 12,010 |
| Protein residues | 1555 |
| Ligands | 0 |
| B-factor (Å2) | 144 |
| RMSD | |
| Bond lengths (Å) | 0.004 |
| Bond angles (°) | 0.584 |
| Validation | |
| Molprobity score | 1.84 |
| Clashscore | 6.82 |
| Poor rotamers (%) | 0 |
| Ramachandran plot | |
| Favored (%) | 92.6 |
| Allowed (%) | 7.4 |
| Outliers (%) | 0 |