Figure 5. The bactofilin cytoskeleton is required to confine the elongasome to the mother cell and bud compartments.

Localization of YFP-RodZ in the H. neptunium wild-type and ΔbacAD backgrounds. Shown are representative cells of strain EC93 (rodZ::eyfp-rodZ) and SP221 (ΔbacAD rodZ::eyfp-rodZ) imaged by DIC and fluorescence microscopy and arranged according to their developmental state. Arrowheads indicate cellular extensions that show YFP-RodZ foci. Bar: 2 µm.

Figure 5—video 1. Localization of YFP-RodZ during stalk synthesis in the wild-type background.

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Cells of strain EC93 (rodZ::eyfp-rodZ) were immobilized on pads made of 1% agarose in ASS medium and imaged at 2 min intervals. Bar: 2 µm.

Figure 5—video 2. Localization of YFP-RodZ during bud formation in the wild-type background.

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Cells of strain EC93 (rodZ::eyfp-rodZ) were immobilized on pads made of 1% agarose in ASS medium and imaged at 2 min intervals. Bar: 2 µm.

Figure 5—video 3. Localization of YFP-RodZ during pseudo stalk synthesis in the ΔbacAD background.

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Cells of strain SP221 (ΔbacAD rodZ::eyfp-rodZ) were immobilized on pads made of 1% agarose in ASS medium and imaged at 2 min intervals. Bar: 2 µm.

Figure 5—video 4. Localization of YFP-RodZ within early stalks in the ΔbacAD background.

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Cells of strain SP221 (ΔbacAD rodZ::eyfp-rodZ) were immobilized on pads made of 1% agarose in ASS medium and imaged at 2 min intervals. Bar: 2 µm.

Figure 5—video 5. Localization of YFP-RodZ in amorphous ΔbacAD cells.

Download video file ^{(1.6MB, mp4)}

Cells of strain SP221 (ΔbacAD rodZ::eyfp-rodZ) were immobilized on pads made of 1% agarose in ASS medium and imaged at 2 min intervals. Bar: 2 µm.