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. 2024 Jan 31;12:RP86577. doi: 10.7554/eLife.86577

Appendix 1—key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Gene (Hyphomonas neptunium) bacA GenBank ABI77372
Gene (Hyphomonas neptunium) bacD GenBank ABI76590
Gene (Hyphomonas neptunium) lmdC GenBank ABI76766
Gene (Rhodospirillum rubrum) bacA GenBank ABC22667
Gene (Rhodospirillum rubrum) lmdC GenBank ABC22668
Strain (Hyphomonas neptunium) LE670 Leifson, 1964 ATCC 15444 Wild-type strain
Strains (Hyphomonas neptunium) LE670 derivatives This paper See Supplementary file 1
Strain (Rhodospirillum rubrum) S1 Molisch, 1907 ATCC 11170 Wild-type strain
Strains (Rhodospirillum rubrum) S1 derivatives This paper See Supplementary file 1Supplementary material 1
Strain (Escherichia coli) BL21(DE3) Invitrogen Cat. #: CMC0016 E. coli B dcm ompT hsdS(rB- mB-) gal
Strain (Escherichia coli) Rosetta(DE3)pLysS Merck Millipore Cat. #: 70956 F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysSRARE (CamR)
Strain (Escherichia coli) TOP10 Thermo Fisher Scientific Cat. #: C404003 F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG
Strain (Escherichia coli) WM3064 Metcalf (unpublished) thrB1004 pro thi rpsL hsdS lacZΔM15 RP4–1360 Δ(araBAD)567 ΔdapA1341::[erm pir(wt)]
Antibody anti-mNeongreen (Mouse monoclonal) Chromotek Cat. #: 32f6 1:1,000
Antibody anti-GFP (Rabbit polyclonal) Sigma Cat. #: G1544 1:10,000
Antibody anti-mCherry (Rabbit polyclonal) BioVision Cat. #: 5993 1:10,000
Antibody anti-HA (Mouse monoclonal) Millipore Cat. #: 05–904 1:1,000
Recombinant DNA reagent pCCFPC-3 (plasmid) Jung et al., 2015 Integrating plasmid for the generation of C-terminal CFP fusions under the control of PCu, RifR
Recombinant DNA reagent pCCFPC-3 derivatives This paper See Supplementary file 1
Recombinant DNA reagent pCCHYC-2 (plasmid) Jung et al., 2015 Integrating plasmid for generation of C-terminal mCherry fusions under control of PCu, KanR
Recombinant DNA reagent pCCHYC-2 derivatives This paper See Supplementary file 1
Recombinant DNA reagent pCCHYC-3 (plasmid) Jung et al., 2015 Integrating plasmid for the generation of C-terminal mCherry fusions under the control of PCu, RifR
Recombinant DNA reagent pCCHYC-3 derivatives This paper See Supplementary file 1
Recombinant DNA reagent pCCHYN-2 (plasmid) Jung et al., 2015 Integrating plasmid for the generation of N-terminal mCherry fusions under the control of PCu, KanR
Recombinant DNA reagent pCCHYN-2 derivatives This paper See Supplementary file 1
Recombinant DNA reagent pCVENC-3 (plasmid) Jung et al., 2015 Integrating plasmid for the generation of C-terminal Venus fusions under the control of PCu, RifR
Recombinant DNA reagent pCVENC-3 derivatives This paper See Supplementary file 1
Recombinant DNA reagent pdCas9-humanized (plasmid) Qi et al., 2013 Addgene plasmid #: 44246; RRID: Addgene_44246 Plasmid carrying a codon-optimized version of the S. pyogenes dCas9 gene
Recombinant DNA reagent pdCas9-humanized derivatives This paper See Supplementary file 1
Recombinant DNA reagent pET21a(+) Novagen Plasmid for the overexpression of C-terminally His6-tagged proteins, AmpR
Recombinant DNA reagent pET21a(+) derivatives This paper See Supplementary file 1
Recombinant DNA reagent pNPTS138 (plasmid) R. K. Alley, unpublished sacB-containing suicide vector used for double homologous recombination, KanR
Recombinant DNA reagent pNPTS138 derivatives This paper See Supplementary file 1
Recombinant DNA reagent pRSFDuet-1 (plasmid) Novagen Plasmid for the coexpression of genes under the control of the T7 promoter
Recombinant DNA reagent pRSFDuet-1 derivatives This paper See Supplementary file 1
Recombinant DNA reagent pRXMCS-2 (plasmid) Thanbichler et al., 2007 Low-copy replicative plasmid for the ectopic expression of genes under the control of Pxyl, KanR
Recombinant DNA reagent pRXMCS-2 derivatives This paper See Supplementary file 1
Recombinant DNA reagent pTB146 (plasmid) Bernhard, unpublished Plasmid for overexpression of N-terminally His6-SUMO-tagged proteins, AmpR
Recombinant DNA reagent pTB146 derivatives This paper See Supplementary file 1
Recombinant DNA reagent pXYFPC-2 (plasmid) Thanbichler et al., 2007 Integrating plasmid for generation of C‐terminal eYFP fusions under control of Pxyl, KanR
Recombinant DNA reagent pXYFPC-2 derivatives This paper See Supplementary file 1
Recombinant DNA reagent pZVENC-2 (plasmid) Jung et al., 2015 Integrating plasmid for the generation of C-terminal Venus fusions under the control of PZn, KanR
Recombinant DNA reagent pZVENC-2 derivatives This paper See Supplementary file 1
Sequence-based reagent DNA oligonucleotides This paper See Supplementary file 1
Peptide, recombinant protein LmdCHne (aa 1-38) GenScript Biotin-MAKWSANLKATFDRAFPERQIYHRSGGTVRYISISPWQ
Chemical compound, drug 2,6-diaminopimelic acid (DAP) Sigma Cat. #: 33240–5 g
Chemical compound, drug ampicillin Carl Roth Cat. #: K029.3
Chemical compound, drug cefalexin Sigma Cat. #: C4895-5G
Chemical compound, drug CuSO4 Carl Roth Cat. #: P024.2
Chemical compound, drug kanamycin Carl Roth Cat. #: T832.3
Chemical compound, drug rifampicin Sigma Cat. #: R3501
Chemical compound, drug ZnSO4 Carl Roth Cat. #: K301.1
Chemical compound hydroxy coumarin-carbonyl-amino-D-alanine (HADA) Kuru et al., 2015 Pulse-labeling of sites of ongoing cell wall biosynthesis
Software, algorithm BLAST Altschul et al., 1990 https://blast.ncbi.nlm.nih.gov/Blast.cgi
Software, algorithm DeepTMHMM Hallgren et al., 2022 https://dtu.biolib.com/DeepTMHMM
Software, algorithm PCOILS Lupas, 1996 https://toolkit.tuebingen.mpg.de/tools/pcoils
Software, algorithm PFAM Mistry et al., 2021 http://pfam.xfam.org
Software, algorithm AlphaFold2 Jumper et al., 2021 https://colab.research.google.com/github/sokrypton/ColabFold/blob/main/AlphaFold2.ipynb
Software, algorithm AlphaFold-Multimer Evans et al., 2022 https://colab.research.google.com/github/sokrypton/ColabFold/blob/main/AlphaFold2.ipynb
Software, algorithm SuperPlotsOfData Goedhart, 2021 https://huygens.science.uva.nl/SuperPlotsOfData
Software, algorithm ImageJ Schneider et al., 2012 https://imagej.net/ij/download.html Plug-ins: JACoP (Bolte and Cordelières, 2006; https://imagej.net/plugins/jacop), MicrobeJ (Ducret et al., 2016; https://www.microbej.com/download-2)
Software, algorithm BacStalk Hartmann et al., 2020 https://drescherlab.org/data/bacstalk/
Software, algorithm SMTracker Oviedo-Bocanegra et al., 2021 https://sourceforge.net/projects/singlemoleculetracker/
Software, algorithm Cell-profiles R package Cameron et al., 2014 https://github.com/ta-cameron/Cell-Profiles