Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Foxb1 | NCBI Gene | Gene ID: 64290 | |
| Genetic reagent (M. musculus) | Foxb1tm1(cre)Gabo | MGI | MGI ID: 3772366 | |
| Genetic reagent (M. musculus) | 129P2-Pvalbtm1(cre)Arbr/J | MGI | MGI ID: 3773708 | |
| Genetic reagent (adeno-associated virus) | AAV5-EF1α-DIO-hChR2(H134R)-eYFP | Addgene | Catalog #: 20298-AAV5 | |
| Genetic reagent (adeno-associated virus) | AAV2-EF1α-DIO-eArchT3.0-eYFP | University of North Carolina, Vector Core | Kind gift from Adamantidis Lab | |
| Genetic reagent (adeno-associated virus) | AAV2-hSyn-DIO-hM3D(Gq)-mCherry | Addgene | Catalog #: 44361-AAV2 | |
| Genetic reagent (adeno-associated virus) | AAV2-hSyn-DIO-hM4D(Gi)-mCherry | Addgene | Catalog #: 44362-AAV2 | |
| Antibody | anti-mCherry (Rabbit polyclonal) | Abcam plc. | product code: ab167453 | IF(1:1000) |
| Antibody | anti-c-Fos (Mouse monoclonal) | Abcam plc. | product code: ab208942 | IF(1:2000) |
| Antibody | anti-GFP (Chicken polyclonal) | Aves Labs, Inc. | Product code: GFP-1020 | IF(1:200) |
| Antibody | anti-mouse IgG (Horse polyclonal, biotinylated) | Vector Laboratories | Product code: BA-2000 | IF(1:200) |
| Antibody | anti-chicken IgG (Donkey polyclonal, Cy2-conjugated) | Jackson ImmunoResearch | Product code: 703-225-155 | IF(1:200) |
| Antibody | anti-Rabbit IgG (Donkey polyclonal, Cy3-conjugated) | Jackson ImmunoResearch | Product code: 711-165-152 | IF(1:200) |
| Peptide, recombinant protein | Streptavidin (Alexa Fluor 647-conjugated) | Jackson ImmunoResearch | Product code: 016-600-084 | IF(1:200) |
| Sequence-based reagent | EGFP-f | This paper | PCR primer | CTC GGC ATG GAC GAG CTG TAC AAG |
| Sequence-based reagent | GAB20 | This paper | PCR primer | CAC TGG GAT GGC GGG CAA CGT CTG |
| Sequence-based reagent | GAB22 | This paper | PCR primer | CAT CGC TAG GGA GTA CAA GAT GCC |
| Chemical compound, drug | DAPI | Life Technologies Corporation | Product code: D1306 | IF(1:5000) |
| Chemical compound, drug | Clozapine | Sigma-Aldrich | Product code: C6305 | |
| Chemical compound, drug | CNO | Sigma-Aldrich | Product code: SML2304 | |
| Software, algorithm | Ponemah Software | DSI | PNM-P3P-CFG | |
| Other | Whole-body barometric plethysmography chambers | DSI | Product code: 601-0001-011 | Used in respiration-related experiments |
| Other | Buxco Bias flow pump | DSI | Product code: 601-2201-001 | Used in respiration-related experiments |
| Other | Buxco differential pressure transducer | DSI | Product code: 600-1114-002 | Used in respiration-related experiments |
| Other | Temperature and humidity probe | DSI | Product code: 600-2249-001 | Used in respiration-related experiments |
| Other | ACQ-7700 USB amplifier | DSI | Product code: PNM-P3P-7002SX | Used in respiration-related experiments |
| Other | Dual LED light source | Prizmatix Ltd. | Product code: 34117 | Used for optogenetic experiments. blue (peak λ=453 nm) and lime green (peak λ=536 nm) |
| Other | 1.5 mm optical fiber | Prizmatix Ltd. | Product code: 34131 | Used for optogenetic experiments. Connecting LED light source to rotary joint |
| Other | Rotary joint | Prizmatix Ltd. | Product code: 43043 | Used for optogenetic experiments. |
| Other | Dual fiber patch cord | Prizmatix Ltd. | Product code: 34115 | Used for optogenetic experiments. 2x500 μm diameter |
| Other | Ceramic cannulas | Prizmatix Ltd. | Product code: 43071 | Used for optogenetic experiments. 1.25 mm outer diameter; 230 μm inner diameter |
| Other | 0.66 NA optical fibers | Prizmatix Ltd. | Used for optogenetic experiments. 200 μm outer diameter. |
|
| Other | 473 nm DPSS laser | Laserglow technologies | Product code: LRS-0473-PFO-00500–01 | Used for optogenetic experiments. |