Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6 OlaHsD | Envigo | WT mouse, male | |
| Strain, strain background (Mus musculus) | P2rx7−/− | Jackson Laboratory | B6.129P2-P2rx7tm1Gab/J | Transgenic mouse, male |
| Antibody | anti-CD16/CD32 (Mouse monoclonal) |
BD Bioscience | Clone 2.4G2, #553142 | FACS (1/100) |
| Antibody | anti-CD3A (Mouse monoclonal) |
BD Bioscience | Clone 145-2611 #551163 |
FACS (1/100) PerCP-Cy5.5 |
| Antibody | Anti NK1.1 (Mouse monoclonal) |
BD Bioscience | Clone PK136 #562864 |
FACS (1/100) PE-CF594 |
| Antibody | CD11c (Mouse monoclonal) |
BD Bioscience | Clone HL3 #558079 |
FACS (1/100) PE-Cy7 |
| Antibody | Ly6G (Mouse monoclonal) |
BD Bioscience | Clone 1A8 #561236 |
FACS (1/100) AF700 |
| Antibody | Ly6C (Mouse monoclonal) |
BD Bioscience | Clone AL-21 #560594 |
FACS (1/100) V450 |
| Antibody | SiglecF (Mouse monoclonal) |
BD Bioscience | Clone E50-2440 #740388 |
FACS (1/50) BV605 |
| Antibody | CD4 (Mouse monoclonal) |
BD Bioscience | Clone RM4-5 #563726 |
FACS (1/100) BV711 |
| Antibody | CD8α (Mouse monoclonal) |
BD Bioscience | Clone 53-6.7 #100741 |
FACS (1/100) BV650 |
| Antibody | Ly6G (Mouse monoclonal) |
BD Bioscience | Clone 1A8 #561236 |
FACS (1/100) AF700 |
| Antibody | Ly6C (Mouse monoclonal) |
BD Bioscience | Clone AL-21 #560594 |
FACS (1/100) V450 |
| Antibody | SiglecF (Mouse monoclonal) |
BD Bioscience | Clone E50-2440 #740388 |
FACS (1/50) BV605 |
| Antibody | CD4 (Mouse monoclonal) |
BD Bioscience | Clone RM4-5 #563726 |
FACS (1/100) BV711 |
| Antibody | CD8α (Mouse monoclonal) |
BD Bioscience | Clone 53-6.7 #100741 |
FACS (1/100) BV650 |
| Antibody | CD45.2 (Mouse monoclonal) |
BD Bioscience | Clone 104 #563686 |
FACS (1/100) BV786 |
| Antibody | Ly6G (Mouse monoclonal) |
BD Bioscience | Clone 1A8 #561236 |
FACS (1/100) AF700 |
| Antibody | Ly6C (Mouse monoclonal) |
BD Bioscience | Clone AL-21 #560594 |
FACS (1/100) V450 |
| Antibody | SiglecF (Mouse monoclonal) |
BD Bioscience | Clone E50-2440 #740388 |
FACS (1/50) BV605 |
| Antibody | CD4 (Mouse monoclonal) |
BD Bioscience | Clone RM4-5 #563726 |
FACS (1/100) BV711 |
| Antibody | CD8α (Mouse monoclonal) |
BD Bioscience | Clone 53-6.7 #100741 |
FACS (1/100) BV650 |
| Antibody | CD45.2 (Mouse monoclonal) |
BD Bioscience | Clone 104 #563686 |
FACS (1/100) BV786 |
| Antibody | CD45 (Mouse monoclonal) |
BD Bioscience | Clone 30-F11 #564279 |
FACS (1/100) BUV395 |
| Antibody | γδ TCR (Mouse monoclonal) |
BD Bioscience | Clone GL3 #553178 |
FACS (1/100) PE |
| Antibody | IFN-γ (Mouse monoclonal) |
BD Bioscience | Clone XMG1.2 #554413 |
FACS (1/100) APC |
| Antibody | IL-17A (Mouse monoclonal) |
BD Bioscience | Clone TC11-18H10 #561718 |
FACS (1/100) AF700 |
| Antibody | I-A/I-E (Mouse monoclonal) |
Biolegend | Clone M5/114.15.2 #107651 |
FACS (1/100) APCfire750 |
| Antibody | CD64 (Mouse monoclonal) |
Biolegend | Clone X54-5/7.1 #139303 |
FACS (1/20) PE |
| Antibody | P2RX7 (Mouse monoclonal) |
Biolegend | Clone 1F11 #148706 |
FACS (1/8) PE |
| Antibody | CD140a (Mouse monoclonal) |
Biolegend | Clone APA5 #135905 |
FACS (1/100) PE |
| Antibody | CD11b (Mouse monoclonal) |
eBiosciences | Clone M1/70 #17-0112-82 |
FACS (1/400) APC |
| Antibody | Foxp3 (Mouse monoclonal) |
eBiosciences | Clone FJK16S #12-5773-82 |
FACS (1/100) PE |
| Antibody | NLRP3 (Mouse monoclonal) |
Adipogen | Clone Cryo-2 # AG-20B-0014 |
WB (1/1000) |
| Antibody | ACTIN BETA (Mouse monoclonal) |
Bio-Rad | #VMA00048 | WB (1/60000) |
| Antibody | IL-18 (Rabbit Polyclonal) |
Biovision | #5180R-100 | WB (1/250) |
| Antibody | IL-18 (Mouse monoclonal) |
BioXcell | Clone YIGIF74-1G7 #BE0237 |
Neutralizing antibody (200 uµg per injection) |
| Commercial assay | Mouse IL-1 beta/IL-1F2 | R&D | #DY401 | ELISA |
| Commercial assay | Mouse IL-18 | MBL | #7625 | ELISA |
| Software | Prism8 |
https://www.graphpad.com RRID:SCR_002797 |
||
| Software | FlowJo v10 |
https://www.flowjo.com RRID:SCR_008520 |
||
| Software | ImageJ 2.1.0 |
https://imageJ.net/ImageJ RRID:SCE_002285 |
||
| Chemical compound | Bleomycin Sulfate |
Sigma-Aldrich | #B5507 | (2.5 U/kg) |
| Chemical compound | Ketamine | VIRBAC | Anesthesia (25 mg/kg) |
|
| Chemical compound | Xylasine | Dechra | Sedaxylan | Anesthesia (2.5 mg/k) |
| Chemical compound | Phorbol 12- myristate 13-acetate | Sigma-Aldrich | # P1585 | 50 ng/ml |
| Chemical compound | Ionomycin | Sigma-Aldrich | # IO634 | 0.5 µg/ml |
| Commercial kit | Golgi Plug | BD Biosciences | # 555028 | 1 µl/ml |
| Commercial kit | Cytofix/Cytoperm | BD Biosciences | # 554722 | |
| Commercial kit | ACK lysis buffer | Gibco | A1049201 | |
| Other | Live Dead | Invitrogen | # L23102 | Use to stain dead cells |
| Commercial kit | Lung dissociation kit | Miltenyi Biotech | #130-095-927 | |
| Commercial kit | Spleen dissociation kit | Miltenyi Biotech | #130-095-928 | |
| Other | HEI3090 | PAM of P2RX7, Douguet et al., 2021 | ||
| Other | H&E staining | Abcam | #ab245880 | Used for histology |
| Other | Picro Sirius Red | Abcam | # ab150681 | Used for histology |