Appendix 1—table 2. Summary of data from mice treated with FK506, including graft outcome (where applicable).

List of experimental mice, including genotype, sex, age at start and end of experiment, initial and final body mass and % change (values that decreased or failed to increase are highlighted in red). Engrafted ChR2⁺ motor neurons were histologically determined to be present in all animals. Age at which ipsilateral hindlimb motor deficits were initially observed in vivo and post-mortem observation of intraneural tumour formation: *, **, and *** denotes small, medium and large tumour size, respectively (see Figure 2E). †Denotes animals that died during the course of treatment.

ID	Genotype	Sex	Age (g)		Body mass (g)		δ BM (%)	Onset (days)	Tumor
			Start	End	Initial	Final
14.1 c	Wild-Type	M	71	93	24.8	26.3	106.0	-	*
14.1 h	Wild-Type	F	71	98	19.1	20.5	107.3	82	-
14.1 a	SOD1G93A	M	71	100	26.5	25.5	96.2	82	**
14.1b	SOD1G93A	M	71	101	25.6	26	101.6	82	**
14.1 g	SOD1G93A	F	71	98	19.4	19.1	98.5	80	*
14B.1a	SOD1G93A	M	57	83	25.2	21	83.3	69	***
14B.1b	SOD1G93A	M	57	101	26.3	27.2	103.4	70	**
14B.1c	SOD1G93A	M	92	121	28.2	28.4	100.7	101	not recorded
14B.2a	SOD1G93A	F	90	124	21.1	22.9	108.5	104	not recorded
14B.2b	SOD1G93A	F	90	124	20.8	22.3	107.2	104	not recorded
14B.2c	SOD1G93A	F	90	127	20.2	22.2	109.9	104	not recorded
14B.2d	SOD1G93A	F	90	120	21.9	22.2	101.4	104	not recorded
14B.2e	SOD1G93A	F	90	117	22.4	20.7	92.4	104	***
13B.1a	SOD1G93A	M	90	113	24.2	21.1	87.2	101	**
13B.2b	SOD1G93A	M	85	112	23.8	23.5	98.7	101	**
Animals listed below underwent FK506 treatment in the absence of intraneural engraftment
20.1d	Wild-Type	F	101	131	19.2	20.95	109.1	Not applicable
20.1 f	Wild-Type	F	101	131	21.3	23.3	109.4
20.1 h	Wild-Type	F	101	131	18.1	21.2	117.1
33.1e	Wild-Type	F	95	128	21.1	22.5	106.6
33.1 f	Wild-Type	F	95	128	22	22.5	102.3
33.1 g	Wild-Type	F	95	128	21.3	21	98.6
33.1b	Wild-Type	M	95	107†	26.7	24	89.9
20.1e	SOD1G93A	F	101	131	17.5	19.8	113.1
20.1 g	SOD1G93A	F	101	131	17	21.2	124.7
33.1 a	SOD1G93A	M	95	111†	25.8	23	89.1
33.1 c	SOD1G93A	M	95	128	24.7	25.8	104.5
33.1d	SOD1G93A	M	95	128	23.2	23.2	100.0