Appendix 1—table 3. Summary of data following in vivo engraftment of ChR2+ motor neurons in SOD1G93A mice.
Table shows a full list of all experimental animals reported in the optical stimulation section of this study, including animal ID, sex, age at start and end of study period, duration of graft, body mass at the start and end of the study period and change in body mass. The table shows the three main cohorts reported in this study, based on type of motor neurons that were engrafted and presence/absence of optical stimulation training. All animals exhibited a positive graft survival, determined by histology and/or acute optical nerve stimulation (ONS) at the experimental end-point; data is shown for 20Hz ONS, which elicited maximal tetanic contractile force.
| 2*ID | 2*Sex | Age (g) | 2*Duration (d) | BM (g) | 2*Δ BM (%) | 2*Max Force (g) | ||
|---|---|---|---|---|---|---|---|---|
| Start | End | Start | End | |||||
| Fast-firing Motor neurons (derived from 7DD dissociated EBs pretreated with MMC) | ||||||||
| 21.1 a | M | 103 | 139 | 36 | 25.2 | 23 | 91.3 | 0.39 |
| 21.1e | M | 103 | 140 | 37 | 23.3 | 21.7 | 93.1 | 0.33 |
| 25A1b | M | 118 | 137 | 19 | 28.3 | 23.2 | 82.0 | 0.55 |
| 25 A.1d | M | 118 | 137 | 19 | 27 | 23.2 | 85.9 | 0.54 |
| 24.2 a | M | 117 | 137 | 20 | 27.2 | 23.6 | 86.8 | 0.57 |
| 25B.1b | M | 109 | 135 | 26 | 24.2 | 21 | 86.8 | 0.47 |
| 25 C.1a | M | 101 | 137 | 36 | 26.3 | 24.5 | 93.2 | 0.88 |
| 21.1 f | F | 103 | 140 | 37 | 20.8 | 19 | 91.3 | 0.43 |
| 22.1d | F | 100 | 120 | 20 | 20.5 | 19.5 | 95.1 | 1.10 |
| 22.1e | F | 100 | 121 | 21 | 23.4 | 20.4 | 87.2 | - |
| 24.2d | F | 114 | 137 | 23 | 19.5 | 18.2 | 93.3 | 0.50 |
| 25B.1c | F | 109 | 135 | 26 | 19.8 | 19.2 | 97.0 | 1.04 |
| 25 C.1d | F | 100 | 122 | 22 | 18.5 | 18.6 | 100.5 | 1.11 |
| 25 C.1f | F | 100 | 138 | 38 | 20.2 | 18.5 | 91.6 | 1.48 |
| Average: | 106.8±7.2 | 133.9±7.2 | 27.1±7.8 | 23.2±0.7 | 21.0±0.9 | 91.1±1.6 | 0.72±0.1 | |
| Slow-firing Motor neurons (derived from 5DD dissociated EBs pretreated with MMC) - untrained | ||||||||
| 28.2d | F | 97 | 142 | 45 | 20.3 | 18.4 | 90.6 | 0.57 |
| 28.2 f | F | 97 | 142 | 45 | 20.2 | 17.8 | 88.1 | 0.63 |
| 28.1b | F | 104 | 136 | 32 | 22 | 19.3 | 87.7 | 0.33 |
| 26.1 a | M | 104 | 143 | 39 | 24.8 | 22.3 | 89.9 | 0.24 |
| 28.2b | M | 97 | 128 | 31 | 26.2 | 23.1 | 88.2 | 1.26 |
| 31.1 c | M | 95 | 137 | 42 | 28 | 23.9 | 85.4 | 0.64 |
| 1.1 a | M | 90 | 125 | 35 | 25.9 | 26 | 100.4 | 1.31 |
| 1.1b | M | 90 | 126 | 36 | 28.9 | 30.1 | 104.2 | 2.24 |
| 35–1 c | M | 93 | 124 | 31 | 26.4 | 25.9 | 98.1 | 0.22 |
| 35-1b | M | 93 | 130 | 37 | 29 | 28.3 | 97.6 | 0.97 |
| 35-1e | M | 93 | 130 | 37 | 23.7 | 24 | 101.3 | 0.46 |
| 40–1 c | M | 95 | 133 | 38 | 24 | 23 | 95.8 | - |
| Average: | 95.7±4.6 | 133±6.9 | 37.3±4.8 | 25.0±0.7 | 23.5±0.9 | 93.9±1.7 | 0.81±0.18 | |
| Slow-firing Motor neurons (derived from 5DD dissociated EBs pretreated with MMC)+optical stimulation training | ||||||||
| 28.2 c | M | 97 | 128 | 31 | 25.6 | 24.4 | 95.3 | 8.96 |
| 1.1 c | M | 90 | 121 | 31 | 23.4 | 22.6 | 96.6 | 6.81 |
| 35-1d | M | 93 | 131 | 38 | 27.4 | 28 | 102.2 | 8.69 |
| 40–1 a | M | 95 | 132 | 37 | 25.1 | 24.2 | 96.4 | 3.60 |
| 38–1 c | F | 93 | 136 | 43 | 20.2 | 19.8 | 98.0 | 10.67 |
| 38.1e | F | 95 | 142 | 47 | 22.4 | 19 | 84.8 | 8.73 |
| 45.1 c | F | 96 | 137 | 41 | 21.6 | 21.4 | 99.1 | 4.99 |
| Average: | 94.1±2.3 | 132.4±6.8 | 38.3±6.0 | 23.7±1.0 | 22.8±1.2 | 96.1±2.2 | 7.49±0.94 | |