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. 2024 Jan 11;12:RP87611. doi: 10.7554/eLife.87611

Figure 2. TcaP is the only phage-inducible chromosomal island-like element (PLE)-encoded factor necessary for directing small capsid assembly, which is required for efficient PLE transduction.

Figure 2.

(A–C) Representative transmission electron micrographs (TEMs) from three independent biological replicates of lysate from ICP1-infected strains of V. cholerae with wild type PLE or PLE∆tcaP, as indicated, carrying either the empty vector (EV) or a vector expressing TcaP (tcaP). Insets are enlarged regions of the images highlighting representative particles, and arrowheads indicate capsid types and sizes, as described in the legend. Scale bars are 100 nm. (D) Quantification of PLE genome transduction for the strain indicated represented as the transduction efficiency relative to wild type PLE with an empty vector (pEV). Each dot represents a biological replicate, bars represent the mean, and error bars show standard deviation. The dotted line indicates an efficiency of 100%.

Figure 2—source data 1. Data used to create Figure 2D.
Figure 2—source data 2. Uncropped micrographs used to create Figure 1A–C.