Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (V. cholerae) | ptcaPPLE1 | This paper | CMB2 | V. cholerae E7946 pKL06 PtacRiboE4-tcaP (PLE1); strepR1, cmR2 |
| Genetic reagent (V. cholerae) | pEV5 | This paper | CMB3 | V. cholerae E7946 pKL06 Ptac RiboE-Empty Vector; strepR, cmR |
| Genetic reagent (V. cholerae) | ptcaPPLE5 | This paper | CMB41 | V. cholerae E7946 pKL06 Ptac RiboE-tcaP (PLE5); strepR, cmR |
| Genetic reagent (V. cholerae) | PLE1∆tcaP pEV | This paper | CMB71 | V. cholerae E7946 PLE1::kanR∆tcaP pKL06 PtacRiboE-EV; strepR, cmR, kanR3 |
| Genetic reagent (V. cholerae) | PLE1∆tcaP ptcaPPLE1 | This paper | CMB73 | V. cholerae E7946 PLE1::kanR∆tcaP pKL06 PtacRiboE-tcaP (PLE1); strepR, cmR, kanR |
| Genetic reagent (Escherichia coli) | pcoat::6xHis, pEV | This paper | CMB548 | E. coli BL21 pETDUET6 Ptac-gp122::6xHis, Ptac-EV |
| Genetic reagent (E. coli) | pcoat::6xHis, ptcaP | This paper | CMB555 | E. coli BL21 pETDUET Ptac-gp122::6xHis, Ptac-tcaP |
| Genetic reagent (E. coli) | pcoat::6xHis, pscaffold | This paper | CMB560 | E. coli BL21 pETDUET Ptac-gp122::6xHis, Ptac-gp124 |
| Genetic reagent (E. coli) | pscaffold-decoration-coat::6xHis, ptcaP | This paper | CMB562 | E. coli BL21 pETDUET Ptac-gp124-gp123-gp122::6xHis, Ptac-tcaP |
| Genetic reagent (E. coli) | pscaffold-decoration-coat::6xHis, pEV | This paper | CMB568 | E. coli BL21 pETDUET Ptac-gp124-gp123-gp122::6xHis, Ptac-tcaP |
| Genetic reagent (V. cholerae) | PLE1 pEV | This paper | CMB577 | V. cholerae E7946 PLE1::kanR pKL06.2 PtacRiboE-EV; strepR, cmR, kanR3 |
| Genetic reagent (V. cholerae) | PLE5 pEV | This paper | CMB579 | V. cholerae E7946 PLE5::kanR pKL06.2 PtacRiboE-EV; strepR, cmR, kanR3 |
| Genetic reagent (V. cholerae) | PLE5 ptcaP PLE1 | This paper | CMB581 | V. cholerae E7946 PLE5::kanR pKL06.2 PtacRiboE-tcaP(PLE1); strepR, cmR, kanR3 |
| Genetic reagent (E. coli) | pscaffold-decoration-coat R223H::6xHis, pEV | This paper | CMB603 | E. coli BL21 pETDUET Ptac-gp124-gp123-gp122R223H::6xHis, Ptac-EV |
| Genetic reagent (E. coli) | pscaffold-decoration-coat R223H::6xHis, ptcaP | This paper | CMB604 | E. coli BL21 pETDUET Ptac-gp124-gp123-gp122R223H::6xHis, Ptac-tcaP |
| Genetic reagent (E. coli) | pcoat R223H::6xHis, pEV | This paper | CMB605 | E. coli BL21 pETDUET Ptac-gp122R223H::6xHis, Ptac-EV |
| Genetic reagent (E. coli) | pcoatR223H::6xHis, ptcaP | This paper | CMB606 | E. coli BL21 pETDUET Ptac-gp122R223H::6xHis, Ptac-tcaP |
| Genetic reagent (E. coli) | pcoat::6xHis, ptcaP; pcoat | This paper | CMB609 | E. coli BL21 pETDUET Ptac-gp122::6xHis, Ptac-tcaP and pCDFDuet Ptac-gp122 |
| Genetic reagent (E. coli) | pcoat E234K::6xHis, pEV | This paper | CMB574 | E. coli BL21 pETDUET Ptac-gp122E234K::6xHis, Ptac-EV |
| Genetic reagent (E. coli) | pcoat E234K::6xHis, ptcaP | This paper | CMB575 | E. coli BL21 pETDUET Ptac-gp122E234K::6xHis, Ptac-tcaP |
| Genetic reagent (E. coli) | pcoat E234K::6xHis, pscaffold | This paper | CMB576 | E. coli BL21 pETDUET Ptac-gp122E234K::6xHis, Ptac-gp124 |
| Genetic reagent (bacteriophage ICP1) | ICP1 wild type | McKitterick et al., 2019 | KSΦ49 | ICP1_2011_Dha_A ∆spacer 9 (accession: MH310933) |
| Genetic reagent (bacteriophage ICP1) | ICP1 coat R223H | This paper | Phage 1, DDΦ78 | ICP1_2011_Dha_A ∆spacer 9 Gp122R223H |
| Genetic reagent (bacteriophage ICP1) | ICP1 coat E234K | This paper | Phage 2, DDΦ79 | ICP1_2011_Dha_A ∆spacer 9 Gp122E234K |
| Gene (bacteriophage ICP1) MH310933 | gp122; coat | N/A | Locus tag: ICP12011A_121 protein accession: AXY82215.1 |
Major capsid protein |
| Gene (bacteriophage ICP1) MH310933 | gp123; decoration | N/A | Locus tag: ICP12011A_122 protein accession: AXY82216.1 |
Capsid decoration protein |
| Gene (bacteriophage ICP1) MH310933 | gp124; scaffold | N/A | Locus tag: ICP12011A_123 protein accession: AXY82217.1 |
Capsid assembly scaffolding protein |
| Gene (V. cholerae) KC152960.1 | orf17; tcaP (PLE1) | N/A | Locus tag: orf17 protein accession: AGG09411.1 | Satellite external scaffolding protein |
| Gene (V. cholerae) CP001236.1 | orf17; tcaP (PLE5_2008) | N/A | Locus tag: VC395_ A0477 Protein accession: ACP11313.1 |
Satellite external scaffolding protein |
| Gene (V. cholerae) CP045718.1 | orf17; tcaP (PLE5_2016) | N/A | Locus tag: GG844_ 03730 Protein accession: QGF30303.1 |
Satellite external scaffolding protein |
| Gene (V. cholerae) Assembly: GCF_007050395.1 |
orf17; tcaP (PLE5_2017) | N/A | Locus tag: DM782_ RS18270 Protein accession: WP_001912195.1 |
Satellite external scaffolding protein |
| Commercial assay or kit | NEBNext Ultra II DNA Library preparation kit | New England Biolabs | NEB #E7645, E7103 | |
| Commercial assay or kit | DNeasy blood and tissue DNA purification kit | QIAGEN | QIAGEN 69506 | |
| Software, algorithm | cryoSPARC | Punjani et al., 2017 | cryoSPARC v4.0.3 | |
| Software, algorithm | RELION | Kimanius et al., 2021 | RELION 4.0 | |
| Software, algorithm | AlphaFold2 | Jumper et al., 2021 | AlphaFold2 | |
| Software, algorithm | Phenix | Liebschner et al., 2019 | Phenix | |
| Software, algorithm | COOT | Emsley et al., 2010 | COOT | |
| Software, algorithm | Pyem | Asarnow et al., 2019 | Pyem |