Figure 5. Evolution of mutations in HLA-DQB1 molecule.

(A) Somatic mutations in single molecules of HLA-DQB1 NM_002123. The position of mutation is indicated at the bottom of the graph. The mutation is numerically numbered from C-terminus to N-terminus. The numbers of cells expressing these mutation isoforms from each cluster (indicated at the top) or sample of origin (indicated at the bottom) are shown in the right panel. Close circle, mutation codon; open circle, wild-type codon; open rectangle, double single-nucleotide mutation in the same codon. (B) Pathway flowchart of mutation accumulation in single molecules of HLA-DQB1. The area of the circle is proportional to the accumulated number of mutations in a molecule. The scale on the left indicates the number of mutations in a single molecule but is not mathematically scaled. The arrow indicates the potential pathways of mutation accumulation in these molecules. The number of white text indicates specific mutation(s) in a molecule. The number of red text indicates the number of cells expressing the mutation(s).